Bio rad western blot imager12/20/2023 ![]() Fluorescence*: Yes (stain-free imaging* and UV-excitable dyes).These “green” gels provide equal or better sensitivity than Coomassie staining and eliminate organic waste disposal problems Bio-Rad stain-free gels are western blot compatible, allowing you to check electrophoresis results and quality prior to western blotting. Stain-free technology - condense your 2 hr Coomassie protocol into a 5 min stain-and-image step with this stain-free gel imaging system.Purchase only what you need and upgrade as your research needs change Clearly defined and color-coded trays eliminate confusion. Modular design and flexible options - use specific trays for different gel imaging applications.Time and space savings - obtain results faster without spending time on various steps in image acquisition and save valuable bench space using this compact system.Get analyzed results including relative molecular weights, bands quantitation, Excel reports, PDFs, and more in a matter of minutes Publication-quality images and full data analysis - get clean and smooth publication-ready images with decreased pixelation when images are cropped or zoomed. ![]() Create a default protocol once, then use the green button on the front of the instrument to use these gel imaging and analysis settings time and again Easy to use - researchers unfamiliar with the Gel Doc EZ imaging system can learn to use it quickly and easily because there is no need for manual control of filters, lens, or lights.Features and Benefits of the Gel Doc EZ System The intuitive Image Lab Software bundled with the Gel Doc EZ Gel Imaging System includes capabilities such as auto image capture, auto analysis, user preferences, and many other features, making gel imaging and analysis incredibly easy. Stain-free tray for stain-free imaging with Bio-Rad stain-free gels.Blue tray for nucleic acid applications with SYBR stains.White tray for Coomassie, copper, silver, and zinc stains.UV tray for ethidium bromide staining of DNA gels and fluorescence imaging.The Gel Doc EZ System provides unparalleled flexibility with the use of four application-specific trays: Prepare 0.The Gel Doc EZ System is a compact and automated gel imaging instrument designed to yield publication-quality images and analyzed results with just the push of a button. Mix the Clarity Western ECL Substrate Kit components in a 1:1 ratio. It is important to use an ECL substrate that has good sensitivity and long signal duration, such as the Clarity Western ECL Substrate. After the final wash step, keep the blot in TBST while preparing for blot detectionĪll PrecisionAb Antibodies were validated using enhanced chemiluminescent (ECL) detection. Rinse the blot with 15 ml TBST at RT for 5 min. Incubate the blot in the secondary antibody and blocking buffer solution at RT for 1 hr with gentle agitation Please refer to the antibody product page for details on the exact secondary antibody used during the validation process. Repeat for a total of five washesĭilute the appropriate secondary antibody in 10 ml blocking buffer according to the following table: Incubate the blot in the primary antibody and blocking buffer solution at 4☌ overnight with gentle agitation Please see the validation protocol (bulletin 6603) for more details.ĭilute the primary antibody 1:1,000 in 10 ml blocking buffer If using BSA, you may notice some nonspecific bands due to its low stringency. We recommend using casein or nonfat dried milk for blocking. When using casein, do not block for longer than 30 min to prevent reduction in signal specificity. Load the control cell lysate adjacent to your samples and the molecular weight (MW) marker (see diagram).ĭuring the validation process, we blocked for 30 min at room temperature (RT) in blocking buffer + 0.1% Tween 20. If using BME, add 180 μl H20, 200 μl 2x Laemmli Sample Buffer, and 20 μl BME If using DTT, add 190 μl H20, 200 μl 2x Laemmli Sample Buffer, and 10 μl 2 M DTT ![]() Reconstitute 400 µg lysate in one of the following ways, depending on the reducing reagent used: Secondary antibodies (see antibody datasheet) Trans-Blot Turbo Mini PVDF Transfer Pack.Transfer membranes, reagents, and equipment ![]()
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